- Device
- Johannes Gutenberg University Mainz
Leica DMi8 S TIRF
- Medicine
- Optical Microscopy
- Device Type
- Optical Microscope
- Device Name
- Leica DMi8 S TIRF
- Device Variants
- Fluorescence Wide-Field Microscope (inverted)
Device Description
The Leica DMi8 S TIRF is an inverted widefield microscope for imaging live and fixed cells. It is equipped with a complete incubation housing system for temperature and CO2 control. An external light source (EL 6000) for conventional fluorescence microscopy and phase contrast imaging is mounted. In addition, it features a TIRF module (= Total Internal Reflection), which is ideal for imaging samples located near the interface between the cover glass and water, such as membrane receptors. In TIRF mode, four lasers are available for excitation (405, 488, 561, 638 nm) and a special objective (100x/1.47 TIRF Oil). The fluorescence emission is captured by a very fast and sensitive Leica DFC9000 GTC sCMOS camera. The following objectives are also available for conventional mode: 5x/0.12 PH0, 20x/0.40 CORR PH1, 40x/0.60 CORR PH2.
Device Application
Time-lapse, high-resolution imaging.
