Rhein-Main Universitäten (RMU)

The Leica TCS SP 8 DIVE is designed for in vivo and in vitro observation of thick tissue material or for applications on small animals. It features a special water immersion objective with motorized correction (IRAPO 25x/1.00 NA Water). An IR laser (Mai Tai HP DeepSee) with an adjustable wavelength from 690 to 1090 nm is used for multiphoton excitation, and a 3-channel reflection light detection system RLD-NDD (1PMT, 2 HyD) is used for image acquisition. For conventional confocal imaging, 2-channel PMT detection and 2 lasers (488 nm, 552 nm) are available. For high-speed imaging, an 8 kHz tandem scanner is used to overcome challenges such as small movements when observing live animals.